Matrix metalloproteinase(MMP) sensitive hyaluronic acid hydrogel-nanoparticle complex와 rhBMP-2를 이용한 골재생

남정훈; 박종철; 유상배; 정용일; 태기융; 김정주; 박용두; 장정원; 이종호

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Matrix metalloproteinase(MMP) sensitive hyaluronic acid hydrogel-nanoparticle complex와 rhBMP-2를 이용한 골재생
BONE REGENERATION WITH MMP SENSITIVE HYALURONIC ACID-BASED HYDROGEL, rhBMP-2 AND NANOPARTICLES IN RAT CALVARIAL CRITICAL SIZE DEFECT(CSD) MODEL

대한구강악안면외과학회지 2009년 35권 3호 p.137 ~ 445

남정훈, 박종철, 유상배, 정용일, 태기융, 김정주, 박용두, 장정원, 이종호,

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남정훈 ( Nam Jeong-Hun ) - 한림대학교 의과대학 강남성심병원 구강악안면외과학교실
박종철 ( Park Jong-Chul ) - 서울대학교 치과대학 구강악안면외과학교실
유상배 ( Yoo Sang-Bae ) - 서울대학교 치과대학 치학연구소
정용일 ( Chung Yong-Il ) - 광주과학기술원 신소재공학과
태기융 ( Tae Gi-Yoong ) - 광주과학기술원 신소재공학과
김정주 ( Kim Jung-Ju ) - 고려대학교 의과대학 의공학교실
박용두 ( Park Yong-Doo ) - 고려대학교 의과대학 한국인공장기센터
장정원 ( Jahng Jeong-Won ) - 서울대학교 치과대학 치학연구소
이종호 ( Lee Jong-Ho ) - 고려대학교 의과대학 의공학교실

KMID : 0355620090350030137

Abstract

As an efficient controlled release system for rhBMP-2, a functional nanoparticle-hydrogel complex, incorporated with matrix metalloproteinase(MMP) sensitive peptide cross-linker, was developed and used as a bone transplant. In vivo bone formation was evaluated by soft x-ray, histology, alkaline phosphatase(ALP) activity and mineral contents analysis, based on the rat calvarial critical size defect(8mm in diameter) model.
Significantly, effective bone regeneration was achieved with the rhBMP-2 loaded MMP sensitive hyaluronic acid(HA) based hydrogel-Nanoparticles(NP) complex, as compared to only MMP HA, the MMP HA-NP without rhBMP-2, or even with the rhBMP-2. These improvements included the formation pattern of bone and functional marrow, the degree of calcium quantification, and the ALP activity.
These results indicate that the MMP sensitive HA with nano-particle complex can be a promising candidate for a new bone defect replacement matrix, and an enhanced rhBMP-2 scaffold.